Molecular and Cellular Biologist

Description

Rangam is seeking candidates for a Contract role as a Molecular and Cellular Biologist with our client Pfizer, one of the world’s largest pharmaceutical companies. Seeking candidates in Cambridge,MA or willing to relocate.

Onsite

Duration: 6-months

POSITION SUMMARY:

We are seeking a scientist to join client’s Research Unit. The successful candidate will be responsible for generating massive single-cell perturbational data using functional genomics and single cell transcriptomics technologies in collaboration with a small team.

POSITION RESPONSIBILITIES:

• Generate custom lentiviral libraries and maintain extensive cell cultures.
• Generate edited pools of cells via lentiviral transduction and arrayed electroporation.
• Generate single-cell data via several commercial workflows, including from 10x Genomics.
• Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale.

ORGANIZATIONAL RELATIONSHIPS:

• Reports to a lead scientist in Lab.
• Collaborates with and supports several multi-disciplinary experimental teams on a per-project basis.

EDUCATION AND EXPERIENCE:

• Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field

TECHNICAL SKILLS REQUIREMENTS:

• 3+ years of hands-on experience with standard cell culture techniques including primary immune cells handling and isolation, maintenance of cell lines and in vitro stimulation of primary immune cells, experience with standard molecular biology techniques including lentivirus infection, qPCR, western blot, and flow cytometry.
• Bonus: direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing.

PHYSICAL POSITION REQUIREMENTS:

• Typical bench work, including sitting, pipetting, lifting <10lb items

Hard Skills:

• Diverse Cell Types
• Molecular biology techniques *qPCR
• Gene Editing

Preferred:

• Direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing.

What will the resource be working with, or, have exposure to?:

• Lentivirus
• Unscreened blood, human tissue or human cell lines

What is the minimum education experience required?: 

• Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field

Sunshine Act

 

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations.  These laws and regulations require Pfizer to provide government agencies with information such as a health care provider’s name, address and the type of payments or other value received, generally for public disclosure.  Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act.  Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government.  If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

 

 

EEO & Employment Eligibility

 

It is the policy of Rangam Consultants, Inc. to provide equal employment opportunities to all applicants and employees without regard to any legally protected status such as race, color, religion, gender, national origin, age, disability or veteran status. 

 

To find out more about Rangam and this role, click the apply button.